Delivery of proteins by intermembrane transfer for...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A01K 67/027 (2006.01) A61K 35/12 (2006.01) A61K 39/00 (2006.01) A61K 47/48 (2006.01) C07K 14/705 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) G01N 33/564 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2144767

GPI-linked proteins are transferred, especially in vivo, from vertebrate carrier cells to cells of a target vertebrate tissue. For example, for purposes of xenogeneic transplantation, cells bearing complement inhibition factor(s) specific for the recipient spe- cies, e.g., genetically engineered, red blood cells, are incubated with transplantable cells of a second, discordant donor species un- til transfer of the factor(s) occurs, The transfer may occur in vivo or in vitro, and the factor-bearing cells may be normal cells of the recipient species, or genetically engineered cells of the donor species which express a gene encoding a complement inhibition factor(s) of the recipient species. The method is particularly useful in modifying pig organs for xenotransplantation into humans.

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