.delta.17 desaturase and its use in making polyunsaturated...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/02 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2663807

The present invention relates to .DELTA.17 desaturases, which have the ability to convert ~-6 fatty acids into their ~-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these .DELTA.17 desaturases in oleaginous yeast are disclosed.

La présente invention concerne des .DELTA.17 désaturases qui sont susceptibles de transformer des acides gras v-6 en leurs homologues v-3 (c'est-à-dire une transformation d'acide arachidonique [20:4, ARA] en acide eicosapentaénoïque [20:5, EPA]). L'invention concerne également des fragments d'acide nucléique isolés et des produits de recombinaison comprenant ces fragments codant pour les .DELTA.17 désaturases, ainsi qu'un procédé destiné à fabriquer des acides gras polyinsaturés à longue chaîne (PUFA) à l'aide de ces .DELTA.17 désaturases, dans une levure oléagineuse.

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