C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 15/09 (2006.01) C12N 15/81 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2717348
Described here are A4 desaturases that convert all- cis- 7,10,13,16,19- docosapentaenoic acid ["DPA"; 22:5 .omega.-3] to docosahexaenoic acid ["DHA"; 22:6 .omega.-3], with secondary activity in converting docosatetraenoic acid ["DTA"; 22:4 .omega.-6] to all- cis-4,7,10,13,16-docosapentaenoic acid ["DPAn-6"; 22:5 .omega.-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding .increment.4 desaturases as well as methods of making long chain polyunsaturated fatty acids ["PUFAs"] using this .increment.4 desaturase in oleaginous yeast.
L'invention concerne des désaturases ?4 qui convertissent l'acide tout-cis-7,10,13,16,19-docosapentaénoïque ["DPA"; 22:5 ?-3] en acide docosahexaénoïque ["DHA"; 22:6 ?-3] ayant une activité secondaire dans la conversion d'acide docosatétraénoïque ["DTA"; 22:4 ?-6] en l'acide tout-4,7,10,13,16-docosapentaénoïque ["DPAn-6"; 22:5 ?-6]. Linvention concerne également des fragments d'acide nucléique isolés et des constructions recombinantes comportant de tels fragments codant pour la désaturase ?4 ainsi que des procédés de fabrication d'acides gras poly-insaturés à longue chaîne ["PUFA"] utilisant cette désaturase ?4 dans une levure oléagineuse.
Xue Zhixiong
Zhu Quinn Qun
E. I. Du Pont de Nemours And Company
Torys Llp
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