.delta.6 desaturases and their use in making polyunsaturated...

Details .delta.6 desaturases and their use in making polyunsaturated... .delta.6 desaturases and their use in making polyunsaturated...

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/81 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2732510

The present invention relates to .DELTA.6 desaturases, which have the ability to convert linoleic acid ["LA"; 18:2 .omega.-6] to .gamma.-linolenic acid ["GLA"; 18:3 .omega.-6] and/or a-linolenic acid ["ALA"; 18:3 .omega.-3] to stearidonic acid ["STA"; 18:4 .omega.-3]. Isolated nu-cleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.6 desaturases, along with methods of mak-ing long-chain polyunsaturated fatty acids ["PUFAs"] using these .DELTA.6 desaturases in oleaginous yeast, are disclosed.

L'invention concerne des ?6 désaturases qui possèdent l'aptitude à convertir de l'acide linoléique ["LA"; 18 : 2 ?-6] en acide ?-linoléique ["GLA"; 18 : 3 ?-6] et/ou de lacide a-linoléique en acide stéaridonique ["STA"; 18:4 ?-3]. Des fragments d'acide nucléique isolés et des constructions recombinantes comportant de tels fragments codant des ?6 désaturases, de même que des procédés de fabrication d'acides gras polyéthyléniques à longue chaîne ["PUFA"] utilisant ces ?6 désaturases dans des levures oléagineuses, sont décrits.

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