Delta 6-desaturases from primulaceae, expressing plants and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2476041

The present invention relates to an improved method for the specific production of unsaturated .omega.-3 fatty acids and a method for the production of triglycerides having an increased content of unsaturated fatty acids, in particular .omega.-3 fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having an increased content of fatty acids, oils or lipids having .DELTA. 6 double bonds due to the expression of a .DELTA. 6-desaturase from Primulaceae. The invention additionally relates to expression cassettes containing a nucleic acid sequence, a vector and organisms containing at least one nucleic acid sequence or an expression cassette. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and use thereof.

La présente invention concerne un procédé amélioré de production spécifique d'acides gras O-3 insaturés et un procédé de production de triglycérides présentant une teneur accrue en acides gras insaturés, en particulier des acides gras O-3 présentant plus de trois liaisons doubles. Cette invention concerne également la production d'un organisme transgénique, de préférence une plante transgénique ou un micro-organisme transgénique, présentant une teneur accrue en acides gras, huiles ou lipides à liaisons doubles ? 6 en raison de l'expression d'une ? 6-désaturase de Primulacées. Ladite invention concerne, en outre, des cassettes d'expression contenant une séquence d'acide nucléique, un vecteur et des organismes contenant au moins une séquence d'acide nucléique ou une cassette d'expression. De plus, cette invention se rapporte à des acides gras insaturés et des triglycérides présentant une teneur accrue en acides gras insaturés ainsi que l'utilisation de ces derniers.

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