C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 1/10 (2006.01) C12N 1/13 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/63 (2006.01) C12N 15/80 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2683497
The present invention relates to .DELTA.8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 .omega.-6) to dihomo-.gamma.-linolenic acid (DGLA; 20:3 .omega.-6) and/or eicosatrienoic acid (ETrA; 20:3 .omega.-3) to eicosatetraenoic acid (ETA; (20:3 .omega.-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding .DELTA.8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these .DELTA.8 desaturases in oleaginous yeast are disclosed.
L'invention concerne des gènes de .DELTA.8 désaturase, capables de convertir l'acide eicosadiénoïque (EDA; 20:2 .omega.-6) en acide dihomo-.gamma.-linolénique (DGLA; 20:3 .omega.-6) et/ou l'acide eicosatriénoïque (ETrA; 20:3 .omega.-3) en acide eicosatétraénoïque (ETA; (20:3 .omega.-3). L'invention se rapporte à des fragments d'acide nucléique isolés et à des produits de recombinaison d'ADN renfermant lesdits fragments qui codent les .DELTA.8 désaturases, et à un procédé de fabrication d'acides gras polyinsaturés à chaîne longue ("polyunsaturated fatty acids" ou PUFA) qui fait appel auxdites .DELTA.8 désaturases dans une levure oléagineuse.
Damude Howard Glenn
Zhu Quinn Qun
E. I. Du Pont de Nemours And Company
Torys Llp
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