.delta.9 elongases and their use in making polyunsaturated...

Details .delta.9 elongases and their use in making polyunsaturated... .delta.9 elongases and their use in making polyunsaturated...

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2682962

The present invention relates to .DELTA.9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ~-6) to eicosadienoic acid (EDA; 20:2 ~-6) and/or a-linolenic acid (ALA; 18:3 ~-3) to eicosatrienoic acid (ETrA; 20:3 ~-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding .DELTA.9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these .DELTA.9 elongases in oleaginous yeast are disclosed.

L'invention se rapporte à des .DELTA.9 élongases pouvant convertir l'acide linoléique (LA; 18:2 ~-6) en acide eicosadiénoïque (EDA; 20:2 ~-6) et/ou l'acide .alpha.-linolénique (ALA; 18:3 ~-3) en acide eicosatriénoïque (ETrA; 20:3 ~-3). Cette invention concerne en outre des fragments d'acide nucléique isolés et des constructions recombinées comprenant ces fragments qui codent des .DELTA.9 élongases, ainsi qu'un procédé de production d'acides gras polyinsaturés (PUFA) à chaîne longue au moyen de ces delta-9 élongases chez une levure oléagineuse.

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