Denaturant-free electrophoresis of biological molecules...

Details Denaturant-free electrophoresis of biological molecules... Denaturant-free electrophoresis of biological molecules...

G - Physics

01

N

G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2407124

The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to eletrophoresis using a matrix that is essentially free of denaturants and having at least one random, linear copolymer comprising a first comonomer of acrylamide and at least one secondary comonomer. A temperature of at least a portion of the matrix is at least about 80°C.

L'invention concerne une méthode permettant de diviser un échantillon comprenant des composés biologiques, par exemple des acides nucléiques. Les acides nucléiques sont soumis à une électrophorèse à l'aide d'une matrice ne contenant essentiellement pas de dénaturants et comprenant au moins un copolymère statistique linéaire comprenant un premier comonomère d'acrylamide et au moins un comonomère secondaire. La température d'au moins une partie de la matrice est d'au moins 80 ·C environ.

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