Denaturant stable and/or protease resistant, chaperone-like...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 9/96 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2497719

Novel denaturant-stable, protease resistant, homo-oligomeric proteins, also referred to herein as stable proteins (SPs), having chaperone-like activity; methods of production and purification of SPs; nucleic acids encoding SPs; methods of isolating nucleic acids encoding SPs; antibodies recognizing SPs; the use of SPs for stabilizing, refolding, repairing, preventing aggregation and de-aggregating macromolecules such as proteins; fusion proteins including SPs; nucleic acid constructs encoding the fusion proteins; and their uses in a variety of methods and applications.

L'invention concerne de nouvelles protéines homo-oligomériques, résistant aux protéases et stables face aux dénaturants, également appelées protéines stables (PS) et dont l'activité est semblable à celle des chaperons. Cette invention concerne également des procédés de fabrication et de purification des PS ; des acides nucléiques codant les PS ; des procédés d'isolation d'acides nucléiques codant les PS ; des anticorps reconnaissant les PS ; l'utilisation des PS pour stabiliser, replier, réparer, empêcher l'agrégation et la désagrégation de macromolécules telles que les protéines ; des protéines de fusion comprenant des PS ; des constructions d'acide nucléique codant les protéines de fusion ; ainsi que leurs utilisations dans une variété de procédés et d'applications.

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