Denatured vehicular proteins to improve enzyme linked...

G - Physics – 01 – N

Patent

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G01N 33/576 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2081657

The present invention pertains to enzyme linked immunoassay methods using a fusion protein as a specific binding pair member of the binding counterpart to be measured. The fusion protein contains a specific binding component and a vehicular component. Non-specific interaction between the specific binding pair partner in the sample to be determined (analysandum) and the vehicular protein is minimized by combining denatured vehicular protein with the analysandum. Various assay methods are taught using the invention.

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