Denaturing gradient affinity electrophoresis and methods of...

Details Denaturing gradient affinity electrophoresis and methods of... Denaturing gradient affinity electrophoresis and methods of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2331767

Methods are described for separation and analysis, and a kit for separation and analysis, of single nucleotide polymorphisms, or mutations, in a target nucleic acid using denaturing gradient electrophoresis through supporting media containing one or more immobilized nucleic acid capture ligand. The method is especially useful for analyzing genetic haplotypes in samples with multiple linked polymorphic sites.

L'invention concerne des méthodes de séparation et d'analyse, ainsi qu'un kit permettant de séparer et d'analyser des polymorphismes ou des mutations d'un nucléotide dans un acide nucléique cible, selon une technique d'électrophorèse par dénaturation selon l'affinité d'un gradient utilisant un milieu de support contenant un ou plusieurs ligands de capture d'acide nucléique immobilisés. La méthode de cette invention est particulièrement utile pour analyser des haplotypes génétiques dans des échantillons avec plusieurs sites polymorphes liés.

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