Dendritic cell vaccine containing telomerase reverse...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/078 (2010.01) C12N 5/0783 (2010.01) C12N 5/0784 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12Q 1/02 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2347067

The invention provides a method of activating a T lymphocyte by contacting the T lymphocyte with a dendritic cell (DC) that presents a telomerase reverse transcriptase (TRT) peptide in the context of a MHC class I or MHC class II molecule. The DC may be pulsed with a TRT polypeptide or may comprise a recombinant polynucleotide encoding a TRT such as hTRT. The invention also provides DCs comprising a recombinant TRT polynucleotide. The methods and compositions of the invention are used in prevention and treatment of cancers and other cell proliferation diseases or conditions.

Cette invention a trait à une technique d'activation de lymphocyte T par sa mise en contact avec une cellule dendritique (DC) qui exprime un peptide de télomérase transcriptase inverse (TRT) dans le contexte d'une molécule de la classe I MHC ou II MHC. Cette cellule dendritique peut être pulsée avec un polypeptide TRT ou peut comporter un polynucléotide de recombinaison codant un TRT tel que TRTh. Cette invention porte également sur des DC renfermant un polynucléotide TRT. On utilise ces compositions et l'on met en oeuvre les techniques afférentes aux fins de la prévention et du traitement du cancer ainsi que d'autres états ou maladies à prolifération cellulaire.

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