Dendritic cells transiently transfected with a membrane...

A - Human Necessities – 61 – K

Patent

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Details

A61K 35/12 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 15/63 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2642999

The invention provides improved methods of producing dendritic cells ("DCs") that transiently express a membrane homing peptide and optionally at least one additional antigen. These DCs have the ability to home to lymph nodes in vivo. In some embodiments, these DCs can be administered to a patient intravenously and can subsequent ly home to lymph nodes and stimulate an immune response. The methods and DCs of the invention are useful for the treatment of various diseases and disorders.

La présente invention concerne des méthodes améliorées de production de cellules dendritiques (<= DC >=) qui expriment de façon transitoire un peptide membranaire de localisation, et éventuellement au moins un antigène supplémentaire. Ces DC ont la capacité de se localiser in vivo au niveau des ganglions lymphatiques. Dans certains modes de réalisation, ces DC peuvent être administrées à un patient par voie intraveineuse, pour ensuite se localiser au niveau des ganglions lymphatiques et stimuler une réponse immunitaire. Les méthodes et DC selon l'invention peuvent être employées dans le traitement de divers troubles et maladies.

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