Deoxyribonucleic acid molecules useful as probes for...

C - Chemistry – Metallurgy – 12 – Q

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195/1.23, 195/1.

C12Q 1/68 (2006.01)

Patent

CA 1240629

ABSTRACT OF THE DISCLOSURE A single-stranded deoxyribonucleic acid molecule having a length of less than about 25 kb comprises at least three distinct nucleotide sequences which are the sites for incorporation into a chromosome of a deoxyribonucleic acid molecule encoding a deleterious gene. Deoxyribonucleic acid probes have been prepared from such molecules and are useful as hybridization probes for detecting chromosomal deoxyribo- nucleic acid which has a deoxyribonucleic acid molecule encoding a deleterious gene incorporated therein. A single-stranded deoxyribonucleic acid molecule derived from human chromosome 22 which is about 5.8 kb in length contains sites for incorporation of a deoxyribonu- cleic acid molecule encoding the oncogene c-ab1 derived from human chromosome 9. Deoxyribonucleic acid probes have been prepared from this molecule and used to detect the abnormal Philadelphia chromosome and chronic myelocytic leukemia.

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