Depletion of endogenous primordial germ cells in avian species

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/873 (2010.01) C12N 5/075 (2010.01) A01K 67/027 (2006.01) A61K 39/00 (2006.01) C12N 5/16 (2006.01) C12N 15/06 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2513744

Methods for modulating primordial germ cell (PGC) numbers and/or development in avians are provided. In one embodiment, the presently disclosed subject matter provides a method for modulating primordial germ cells numbers in an avian embryo comprising immunizing a female bird with an antigen associated with primordial germ cells, whereby an egg produced by the female bird comprises a sufficiently high concentration of antibodies specific for the antigen to modulate numbers of endogenous PGCs in an avian embryo present within in the egg. Also provided are methods for producing chimeric avians, methods for increasing the proportion of male birds in a plurality of eggs, methods of producing avian gametes, and methods for enhancing germ line transmission of nucleic acids in birds.

L'invention concerne des procédés de modulation du nombre de cellules germinales embryonnaires (PGC) et/ou leur développement dans des oiseaux. Dans l'un des modes de réalisation, l'invention concerne un procédé de modulation du nombre de cellules germinales embryonnaires dans un embryon aviaire consistant à immuniser un oiseau femelle à l'aide d'un antigène associé aux cellules germinales embryonnaires, un oeuf produit par l'oiseau femelle comprenant une concentration suffisamment élevée d'anticorps spécifique à l'antigène en vue de moduler le nombre de PGC endogènes dans un embryon aviaire présent à l'intérieur de l'oeuf. L'invention concerne également des procédés de production d'oiseaux chimères, des procédés d'augmentation de la proportion des oiseaux mâles dans plusieurs oeufs, des procédés de production de gamètes aviaires, et des procédés d'amélioration de la transmission d'une lignée germinale d'acides nucléiques dans des oiseaux.

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