Derivation of terminally differentiated dopaminergic neurons...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0793 (2010.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01) A61K 35/30 (2006.01) A61P 25/28 (2006.01)

Patent

CA 2518577

The present disclosure is directed to improved methods for efficiently producing neuroprogenitor cells and differentiated neural cells such as dopaminergic neurons and serotonergic neurons from pluripotent stem cells, for example human embryonic stem cells. Using the disclosed methods, cell populations containing a high proportion of cells positive for tyrosine hydroxylase, a specific marker for dopaminergic neurons, have been isolated. The neuroprogenitor cells and terminally differentiated cells of the present disclosure can be generated in large quantities, and therefore may serve as an excellent source for cell replacement therapy in neurological disorders such as Parkinson's disease.

L'invention concerne des méthodes améliorées pour produire efficacement des cellules progénitrices nerveuses et des cellules nerveuses différentiées telles que les neurones dopaminergiques et les neurones sérotonergiques à partir de cellules souches pluripotentes, par exemple les cellules souches embryonnaires humaines. On a isolé des populations de cellules contenant une proportion élevée de cellules qui réagissent positivement à la tyrosine hydroxilase, un marqueur spécifique aux neurones dopaminergiques, en utilisant les méthodes décrites. Les cellules progénitrices nerveuses et les cellules à différentiation terminale selon l'invention peuvent être générées en grandes quantités et peuvent donc servir comme une excellente source de cellules dans une thérapie de remplacement cellulaire de troubles neurologiques tels que la maladie de Parkinson.

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