C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.18, 195/1.
C12N 15/00 (2006.01) C07K 14/61 (2006.01) C07K 14/62 (2006.01) C07K 14/65 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 15/71 (2006.01)
Patent
CA 1253092
ABSTRACT The present invention comprises improved deri- vatives of the tryptophan operon useful for expressing fused gene products in E. coli and related organisms. Two of the improved derivatives disclosed are encoded on 0.43 and 0.55 kb EcoRI restriction fragments from plasmids pCZ20 and pLEBGH2 (NRRL B-15881 and NRRL B-15882), respectively. The modified derivatives have been placed on recombinant DNA cloning and expression vectors. A variety of expression vectors have been constructed that drive expression of fused gene prod- ucts. Two novel gene sequences, encoding insulin-like growth factors I and II, have been expressed with the modified tryptophan operon system.
491359
Belagaje Rama M.
Epp Janet K.
Hoskins Jo Ann
Hsiung Hansen M.
Long George L.
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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