Derivatized glass supports for peptide and protein sequencing

Details Derivatized glass supports for peptide and protein sequencing Derivatized glass supports for peptide and protein sequencing

G - Physics

01

N

150/19

G01N 33/68 (2006.01) C07K 1/12 (2006.01) G01N 33/552 (2006.01)

Patent

CA 1339251

ABSTRACT Peptides or proteins are sequenced by stepwise degradation while immobilized on a glass support derivatized with a silica-binding substance bearing a free acid group, especially a sulfonic acid group. The support is preferably derivatized with 2-(4-chlorosulfonyl phenyl) ethyl trimethoxysilane. Peptide sequencing performance is improved if the support is also derivatized with a monomeric silica-binding substance bearing a free quaternary ammonium group, such as n-trimethyoxysilyl propyl-N,N,N-trimethyl ammonium chloride.

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