Desaturase genes and their use

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) A61K 38/44 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01) C12Q 1/26 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2311472

cDNA encoding C. elegans .DELTA.6 desaturase has been cloned and sequenced, and the .DELTA.6 desaturase amino acid sequence has been determined. The C. elegans .DELTA.6 desaturase has a surprisingly low level of sequence identity with the known borage .DELTA.6 desaturase. The C. elegans .DELTA.6 desaturase has been expressed in yeast. It and other desaturases can be cloned in host organisms (e.g. plants) and can be used to provide useful metabolites.

ADNc codant la désaturase de C.elegans .DELTA.?6¿ a été clôné et mis en séquence, avant de déterminer la séquence d'acide aminé de désaturase .DELTA.?6¿. La désaturase de C.elegans .DELTA.?6¿ présente un niveau d'identité de séquence très faible avec la désaturase .DELTA.?6¿ de bourrache. La désaturase de C.elegans .DELTA.?6¿ a été exprimée dans la levure. Cette désaturase, ainsi que d'autres désaturases, peuvent être clonées dans des organismes hôtes (par exemple, des plantes) et permettent d'utiliser des métabolites utiles.

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