C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) C12N 1/10 (2006.01) C12N 1/12 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/12 (2006.01) C12N 15/30 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2354672
The amino acid and nucleic acid sequences of a .DELTA.5-desaturase enzyme and a .DELTA.8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
L'invention concerne les séquences d'acides aminés et d'acides nucléiques d'une enzyme .DELTA.?5¿-désaturase et d'une enzyme .DELTA.?8¿-désaturase. Les séquences d'acides nucléiques peuvent s'utiliser pour mettre au point des produits de recombinaison et des vecteurs d'ADN recombiné. Ces vecteurs peuvent ensuite s'utiliser pour transformer plusieurs organismes, y compris par exemple, des végétaux et de la levure. Les organismes transformés produisent ensuite des acides gras polyinsaturés. Les séquences d'acides aminés s'utilisent pour générer des anticorps spécifiques aux enzymes permettant d'identifier les désaturases.
Browse John A.
Wallis James G.
Watts Jennifer L.
Smart & Biggar
Washington State University Research Foundation
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