Desaturases and process for the production of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C07K 16/40 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2694661

The invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruben, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale that code desaturases and that can be used for the recombinant production of polyunsaturated fatty acids. The invention also relates to the vectors, host cells and transgenic non-human organisms that contain said inventive polynucleotides, and also the polypeptides coded by said polynucleotides. The invention further relates to antibodies against the inventive polypeptides. Finally, the invention relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to the use thereof as medicments, cosmetics, food, animal food, preferably fish food or food supplements.

La présente invention concerne des polynucléotides de Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruben, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii et Microdochium nivale, qui codent des désaturases et qui peuvent être utilisés pour produire par recombinaison des acides gras polyinsaturés. L'invention concerne également des vecteurs, des cellules hôtes et des organismes transgéniques non humains qui contiennent lesdits polynucléotides, ainsi que les polypeptides codés par lesdits polynucléotides. Elle concerne aussi des anticorps dirigés contre lesdits polypeptides. En outre, l'invention concerne des procédés de production d'acides gras polyinsaturés, de compositions de graisses, de lipides et d'acides gras, ainsi que leur utilisation comme médicaments, produits cosmétiques, aliments, aliments pour animaux, de préférence aliments pour poissons, ou comme compléments alimentaires.

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