Design and construction of diverse synthetic peptide and...

C - Chemistry – Metallurgy – 40 – B

Patent

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C40B 50/02 (2006.01) C07K 16/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2664681

The present invention concerns the design and construction of diverse peptide and polypeptide libraries. In particular, the invention concerns methods of analytical database design for creating datasets using multiple relevant parameters as filters, and methods for generating sequence diversity by directed multisyntheses oligonucleotide synthesis. The present methods enable the reduction of large complex annotated databases to simpler datasets of related sequences, based upon relevant single or multiple key parameters that can be individually directly defined. The methods further enable the creation of diverse libraries based on this approach, using multisynthetic collections of discrete and degenerate oligonucleotides to capture the diverse collection of sequences, or portions thereof.

La présente invention concerne la conception et la construction de diverses banques de peptides et de polypeptides. Plus particulièrement, l'invention concerne des procédés de conception de bases de données analytiques pour la création d'ensembles de données au moyen de multiples paramètres pertinents utilisés comme filtres, ainsi que des procédés de génération d'une diversité de séquence par synthèse d'oligonucléotides de type multisynthèse dirigée. Les présents procédés permettent de réduire les bases de données annotées complexes de grande taille en ensembles de données plus simples de séquences associées, sur la base de paramètres clés uniques ou multiples pertinents pouvant être définis directement et individuellement. Lesdits procédés permettent en outre de créer diverses banques sur la base de cette approche, au moyen de collections multisynthétiques d'oligonucléotides discrets et dégénérés, en vue de la capture d'une collection variée de séquences, ou de parties correspondantes.

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