Designed armadillo repeat proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2700391

The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.

L'invention porte sur des collections de protéines de liaison mises au point spécifiques à des cibles à base de protéines de répétition de tatou et sur un procédé de génération de ces collections. Des protéines de répétition de tatou mises au point sont basées sur des séquences consensus d'unités de répétition de tatou individuelles qui peuvent être utilisées comme échafaudages pour la reconnaissance peptidique. Un tel échafaudage fournit un mode de liaison qui est, en principe, le même pour chaque petite unité reconnaissable, par exemple un acide aminé ou un dipeptide, ce qui permet une reconnaissance précise et modulaire d'un peptide en conformation étendue. Le procédé permet de générer une série de modules reconnaissant ces unités simples et de combiner de tels blocs de construction pour créer un site de liaison pour n'importe quelle cible de peptide désirée sans effectuer de sélections supplémentaires.

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