G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 21/76 (2006.01) C12Q 1/42 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2043631
Immobilized macromolecules such as proteins or nucleic acid sequences are tagged with labels which induce a chemiluminescent reaction in a liquid-phase substrate. As the reaction is occurring, the substrate is exposed to a phosphor screen which absorbs the chemiluminescent emission in an image of the pattern formed by the macromolecules. The absorbed energy is then released and read, providing qualitative and optionally quantitative information regarding the immobilized macromolecules.
Des macromolécules immobilisées, telles que des protéines ou des séquences d'acides nucléiques, sont marquées avec des étiquettes qui induisent une réaction chimiluminescente dans un substrat en phase liquide. Pendant que se produit la réaction, le substrat est exposé à un écran fluorescent qui absorbe l'émission chimiluminescente dans une image modelée par les macromolécules. L'énergie absorbée est ensuite libérée et lue, fournissant ainsi de l'information qualitative et, au choix, quantitative sur les macromolécules immobilisées.
Bers George
Witney Franklin R.
Bio-Rad Laboratories Inc.
Fetherstonhaugh & Co.
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