Detecting human antibodies in non-human serum

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2592254

The present invention provides for the quantification of a human, human~chimeric, humanized antibody, or a fragment thereof, without the necessity of using a target-specific molecule. More particularly, the invention relates to a quantification assay that includes the step of blocking non-specific binding sites of a capture reagent with a blocking buffer containing a non-human mammalian globulin, such as bovine gamma globulin (BGG).

La présente invention concerne la quantification d~un anticorps humain, humain chimérique, humanisé, ou d~un fragment de celui-ci, sans qu~il ne soit nécessaire d'utiliser une molécule à spécificité de cible. La présente invention concerne plus particulièrement un test de quantification qui comprend l~étape qui consiste à bloquer les sites de liaison non spécifiques d~un réactif de capture avec un tampon bloquant contenant une globuline de mammifère non humain, telle que la gamma globuline bovine (BGG).

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