Detection and determination of solid phase-associated factors

Details Detection and determination of solid phase-associated factors Detection and determination of solid phase-associated factors

G - Physics

01

N

G01N 33/554 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2256087

The present invention relates to procedures for the detection or for the determination of solid phase-associated factors, which are multiply associated with the same solid phase. According to the invention, the sample is brought into contact with a transmitter particle, on which at least one ligand having binding affinity for a solid phase-associated factor and a transmitter are immobilized, and a receiver particle, on which at least one ligand having binding affinity for said solid phase- associated factor and a receiver is immobilized, and then the signal is determined which results when transmitter and receiver are brought sufficiently close to one another. In particular, the invention relates to the detection of cell surface receptors which can be used for the typing of cells or for the determination of cell activation states. It is thus possible to replace the hitherto widely customary flow cytometry by a more simple procedure.

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