Detection and differentiation of coxiella burnetti in...

C - Chemistry – Metallurgy – 07 – H

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195/1.22, 150/8.

C07H 21/00 (2006.01) C07K 14/29 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1298797

ABSTRACT OF THE DISCLOSURE Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labelled DNA probe. Radio- isotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

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