Detection and identification of human papillomavirus by pcr...

Details Detection and identification of human papillomavirus by pcr... Detection and identification of human papillomavirus by pcr...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01)

Patent

CA 2302146

The present invention relates to a method for detection and/or identification of HPV present in a biological sample, comprising the steps of amplification of HPV polynucleic acids and of hybridization of said amplified polynucleic acids to a number of probes. By means of PCR, a short fragment of the L1 gene of HPV is amplified. The amplimers are then contacted with probes that specifically hybridize to said short fragment of the L1 gene of either one or more than one HPV type. A preferred format is the reverse hybridization technique, more particularly the LiPA technique. The invention also relates to primers and probes to be used in a method of detection and/or identification of HPV and to a diagnostic kit to perform said method.

La présente invention concerne un procédé de détection et/ou d'identification du virus du papillome humain présent dans un échantillon biologique, ce procédé comprenant les étapes consistant à amplifier les acides polynucléiques du virus du papillome humain et à hybrider ces acides polynucléiques amplifiés sur un certain nombre de sondes. Au moyen d'une PCR, on amplifie un court fragment du gène L1 dudit virus, puis on met en contact les amplimères avec les sondes, lesquelles s'hybrident de manière spécifique sur ledit fragment du gène L1 qui est soit d'un type de virus du papillome humain, soit de plusieurs types de ce virus. La technique d'hybridation inverse constitue une méthode préférée, et notamment la technique LiPA. L'invention concerne également des amorces et sondes à utiliser dans un procédé de détection et/ou d'identification du virus du papillome humain, ainsi qu'une trousse diagnostique servant à l'exécution de ce procédé.

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