Detection and quantification of biomolecules using mass...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C40B 30/04 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2671864

The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and release labels for detection by mass spectrometry. This process is easily incorporated into a PCR amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

La présente invention concerne en partie un procédé pour la détection d'un acide nucléique cible à l'aide d'oligonucléotides détecteurs, détectables par spectrométrie de masse. Ce procédé utilise l'activité nucléase de 5' à 3' d'une polymérase d'acide nucléique pour cliver des sondes oligonucléotidiques annelées à partir de duplex hybridés et pour libérer des marqueurs pour une détection par spectrométrie de masse. Ce procédé est facilement incorporé dans un dosage à amplification par PCR. Le procédé comprend également des modes de réalisation se rapportant à une analyse quantitative d'acides nucléiques cibles.

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