Detection and quantification of micro-organisms using...

Details Detection and quantification of micro-organisms using... Detection and quantification of micro-organisms using...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2367587

In livestock a number of pathological conditions and/or syndromes have been noticed that do not seem to be attributable to e.g. an infection with a single micro-organism. These syndromes however do seem to have an effect on the equilibrium which normally exists within the different bacteria constituting the bacterial flora. A number of these syndromes are referred to as dysbacteriosis and bacterial overgrowth. To enable at least in part the evaluation of the "general" health of the livestock the invention provides means and methods for analysing the composition of a microbiological flora. In one aspect a method of the invention comprises providing a sample of said flora, selectively amplifying nucleic acid present in said sample, subjecting the amplificate to restriction digestion and analysing the resulting pattern of restriction fragments.

On a remarqué chez les animaux d'élevage un certain nombre de pathologies et/ou syndromes qui ne semblent pas pouvoir être attribués, par exemple, à une infection par un seul micro-organisme. Ces syndromes semblent cependant affecter l'équilibre existant normalement parmi les différentes bactéries constituant la flore bactérienne. Un certain nombre de ces syndromes est dénommé dysbactériose ou prolifération bactérienne. Afin de pouvoir évaluer en partie l'état général de santé des animaux d'élevage, l'invention concerne des moyens et procédés d'analyse de la composition d'une flore microbiologique. Dans un de ses aspects, l'invention concerne un procédé consistant à prélever un échantillon de cette flore, à amplifier de manière sélective un acide nucléique présent dans l'échantillon, à soumettre le produit amplifié à une digestion par restriction et à analyser le motif résultant des fragments de restriction.

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