C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.3, 150/8.5
C12Q 1/25 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2003501
28 Abstract Detection and Quantitative Determination of RNA and DNA The method for the detection and/or quantitative determination of target RNA or DNA in an analyte sample includes the steps of: a) contacting the analyte with magnetic particles carrying a single stranded 5'-attached DNA probe capable of binding to said RNA or DNA; b) contacting the magnetic particles with an aqueous solution of a reverse transcriptase or polymerase in the presence of labelled nucleotides using the probe as a primer whereby labelled complementary single stranded DNA is formed if said RNA or DNA was present in said analyte; magnetically aggregating the magnetic particles onto a surface and removing said solution; and c) detecting the presence or absence of said label and/or determining the amount of said label. The chain length of complementary single stranded DNA is limited by, for example a predetermined amount of dideoxynucleotides being present at step (b) to limit chain synthesis to a predetermined average length or the probe binding to said DNA or RNA a predetermined distance from a chain terminating point of said DNA or RNA. A kit for preforming the method is disclosed.
Hornes Erik
Korsnes Lars
Dynal A.s.
Kirby Eades Gale Baker
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