Detection and speciation of campylobacter

Details Detection and speciation of campylobacter Detection and speciation of campylobacter

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2168648

Method for detecting Campylobacter by PCR detection of DNA sequence, highly conserved between species lari, coli jejuni and upsaliensis, Speciation between these four is possible as the PCR product is differentially cleaved by restriction endonucleases.

L'invention se rapporte à un procédé de détection du campylobacter par détection d'une séquence d'ADN par amplification PCR, cette séquence étant observée fréquemment parmi les espèces lari, coli, jejuni et upsaliensis. La différentiation entre ces quatre espèces est rendue possible puisque le produit de la PCR est clivé différemment par des endonucléases de restriction.

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