Detection and typing of the icea gene of helicobacter pylori

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/205 (2006.01)

Patent

CA 2306254

The present invention relates to a method for detection and/or typing of alleles of the iceA gene of H. pylori present in a biological sample, with said method comprising: i) amplifying polynucleic acids of the iceA gene by use of primers that permit specific amplification either of a fragment of iceA1 variants or of a fragment of iceA2 variants, and/or ii) hybridizing polynucleic acids of the iceA gene, possibly after amplification as in step i), with at least one probe that specifically hybridizes to either iceA1 variants or to iceA2 variants. In order to enable this method, the present invention discloses nucleic acid sequences of iceA1 and iceA2 variants. Primers and probes that can be used to carry out said method are also disclosed. The present invention further relates to diagnostic kits that allow to perform said method. IceA1 and IceA2 protein sequences are also disclosed, as well as antibodies directed against these sequences.

La présente invention concerne un procédé de détection et/ou de typage d'allèles du gène iceA de H. pylory présent dans un échantillon biologique, consistant à: i) amplifier des acides polynucléiques du gène iceA par utilisation d'amorces permettant une amplification spécifique d'un fragment de variants de iceA1 ou d'un fragment de variants de iceA2, et/ou ii) à hybridiser des acides polynucléiques du gène iceA, éventuellement après une amplification tel qu'indiqué dans l'étape (i), avec au moins une sonde qui réalise une hybridation, de manière spécifique, avec des variants de iceA1 ou des variants de iceA2. Afin de mettre ce procédé en oeuvre, la présente invention concerne des séquences d'acide nucléique de variants iceA1 et iceA2. La présente invention concerne également les amorces et les sondes utilisées pour réaliser cette invention. La présente invention, concerne, en outre, des kits de diagnostic permettant de mettre en oeuvre la présente invention. La présente invention concerne également des séquences de protéines iceA1 et iceA2 ainsi que des anticorps dirigés contre ces séquences.

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