Detection assays having chromogenic and nonchromogenic,...

C - Chemistry – Metallurgy – 12 – Q

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150/15, 150/15.2

C12Q 1/46 (2006.01) C12Q 1/00 (2006.01) C12Q 1/60 (2006.01) G01N 21/31 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2037562

ABSTRACT OF THE INVENTION The invention relates to enzyme detection assay compositions and methods which have chromogenic and nonchromogenic, hypochromogenic, bathochromogenic or hypsochromogenic substrates. By using both chromo- genic and nonchromogenic, hypochromogenic, batho- chromogenic or hypsochromogenic substrates assay sensitivity can be controlled and the need for sample predilution eliminated. In one embodiment of the invention an assay for the estimation of serum cholinesterase activity is provided.

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