Detection conjugate and method for polychromatic analysis

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2747059

The invention provides a combination of reagents as a test kit containing a detection conjugate having a binding portion and a reagent for deactivating of the fluorochrome portion by interaction with the linker. The binding portion especially is an antibody portion. The detection conjugate has a fluorochrome portion connected to the antibody portion, and a linker connected to the fluorochrome portion, wherein the linker comprises an oligonucleotide. The fluorochrome portion can be deactivated by hydrolysis of the linker or by specific hybridization of a quencher having an oligonucleotide to the linker.

L'invention concerne un ensemble de réactifs sous forme de kit d'essai, ledit ensemble renfermant un conjugué de détection présentant une fraction de liaison et un réactif destiné à rendre inefficace la fraction fluorochrome par interaction avec le linker. La fraction de liaison est, en particulier, une fraction d'anticorps. Le conjugué de détection présente une fraction fluorochrome liée avec la fraction d'anticorps, et un linker lié avec la fraction fluorochrome, ledit linker comprenant un oligonucléotide. Par hydrolyse du linker ou par hybridation spécifique d'un quencher avec un oligonucléotide avec le linker, la fraction fluorochrome peut être rendue inefficace.

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