Detection device and method

G - Physics – 01 – N

Patent

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G01N 33/558 (2006.01) G01N 27/02 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2205372

The present invention provides an analyte detection device. The device comprises first and second zones, means to allow addition of a probe to the first zone, means to allow addition of a sample suspected to contain an analyte and means to allow passage of the probe from the first zone to the second zone. The first zone contains ligands reactive with the analyte and the second zone includes a membrane the impedance of which is dependent on the presence or absence of the probe and means to measure the impedance of the membrane. It is preferred that the probe includes an ionophore, preferably gramicidin. The present invention also relates to methods of detecting the presence of an analyte.

La présente invention concerne un dispositif de détection d'analyte. Ce dispositif comporte des première et deuxième zones, des moyens permettant l'addition d'une sonde à la première zone, des moyens permettant l'addition d'un échantillon soupçonné de contenir un analyte, et des moyens permettant le passage de la sonde de la première zone à la deuxième zone. La première zone contient des ligands réactifs avec l'analyte et la deuxième zone comprend une membrane dont l'impédance est fonction de la présence ou de l'absence de la sonde, et des moyens pour mesurer l'impédance de cette membrane. La sonde renferme de préférence un ionophore, idéalement de la gramicidine. La présente invention concerne également des procédés pour détecter la présence d'un analyte.

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