C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/113 (2010.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/06 (2006.01)
Patent
CA 2504911
The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
L'invention concerne un procédé de détection et/ou d'identification spécifiques de l'espèce staphylocoque, notamment le staphylocoque doré, à l'aide de nouvelles séquences d'acide nucléique dérivées de région ITS (Internal Transcribed Spacer = espaceur transcrit interne). Ladite invention concerne également lesdites séquences d'acide nucléique dérivées de la région ITS, entre l'acide ribonucléique ribosomal (ARNr) 16S et 23S ou les gènes d'ARNr, à utiliser pour la détection et/ou l'identification spécifiques de l'espèce staphylocoque, notamment le staphylocoque doré, dans un échantillon biologique. L'invention concerne par ailleurs des amorces d'acide nucléique à utiliser pour l'amplification de ladite région d'espaceur de l'espèce staphylocoque dans un échantillon.
Claeys Sofie
Emrich Thomas
Haberhausen Gerd
Jannes Geert
Verdoodt Lia
Innogenetics N.v.
Roche Diagnostics Gmbh
Smart & Biggar
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