C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2553270
The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and/or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.
L'invention concerne de nouvelles séquences d'acide nucléique dérivées de la région des ITS comprise entre les acides ribonucléiques ribosomaux (ARNr) ou gènes d'ARNr 16S et 23S, servant à la détection et/ou à l'identification spécifiques d'une espèce Proteus, en particulier de <i>Proteus mirabilis, Proteus vulgaris </i>et/ou <i>Proteus penneri, </i> dans un échantillon biologique. L'invention concerne également à un procédé permettant la détection et/ou l'identification spécifiques d'une espèce <i>Proteus </i>, en particulier de <i>Proteus mirabilis, Proteus vulgaris </i>et/ou <i>Proteus penneri, </i> au moyen desdites nouvelles séquences d'acide nucléiques dérivées de la région des ITS (espaceurs internes transcrits). L'invention concerne également des amorces d'acide nucléique servant à l'amplification de ladite région ITS des espèces <i>Proteus </i> dans un échantillon.
Emrich Thomas
Haberhausen Gerd
Jannes Geert
Mijs Wouter
Innogenetics N.v.
Roche Diagnostics Gmbh
Smart & Biggar
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