Detection, identification and differentiation of serratia...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2578401

The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23 S rRNA genes, to be used for the specific detection and/or identification of Serratia species, in particular of Serratia marcescens, Serratia ficaria and/or Serratia fonticola, in a biological sample. The present invention relates also to a method for the specific detection and/or identification of Serratia species, in particular Serratia marcescens, Serratia ficaria and/or Serratia fonticola, using said new nucleic acid sequences derived from the ITS region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Serratia species in a sample.

L'invention concerne de nouvelles séquences d'acides nucléiques dérivées de la région ITS (espaceur transcrit interne) entre les gènes de l'ARNr 16S et 23S à utiliser pour détecter et identifier spécifiquement des espèces du genre Serratia, en particulier, Serratia marcescens, Serratia ficaria et/ouSerratia fonticola, dans un échantillon biologique. L'invention concerne également un procédé permettant de détecter et d'identifier spécifiquement des espèces du genre Serratia, en particulier, Serratia marcescens, Serratia ficaria et/ou Serratia fonticola, à l'aide des nouvelles séquences d'acides nucléiques dérivées de la région ITS. L'invention concerne enfin des amorces d'acides nucléiques à utiliser pour amplifier la région d'espaceur des espèces du genre Serratia dans un échantillon.

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