Detection method and determination method for detection target

G - Physics – 01 – N

Patent

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G01N 33/531 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2710586

A detection/determination kit with which a substance to be detected or the amount thereof can be rapidly, inexpensively, and easily detected or determined; and a detection/determination method. The kit, which is for detecting a substance to be detected (50) contained in a specimen, comprises: a first compound (10) comprising a first substance including a stimulus-responsive polymer (11) and, bonded to the first substance, a first antibody (13) against the substance (50); and a second compound (20) comprising a second substance (21) which is hydrophilic and, bonded thereto, a second antibody (23) against the substance (50). The first antibody (13) and second antibody (23) can simultaneously combine with the substance (50) at different sites in the substance (50).

L'invention concerne un nécessaire de détection/détermination grâce auquel une substance à détecter ou une quantité de celle-ci peut être rapidement, économiquement et facilement détectée ou déterminée, et un procédé de détection/détermination. Le nécessaire, qui peut être destiné à détecter une substance à détecter (50) contenue dans un échantillon, comprend : un premier composé (10) comprenant une première substance contenant un polymère répondant à un stimulus (11) et, lié à la première substance, un premier anticorps (13) contre la substance (50) ; et un second composé (20) comprenant une seconde substance (21) qui est hydrophile, et lié à celle-ci, un second anticorps (23) contre la substance (50). Le premier anticorps (13) et le second anticorps (23) peuvent se combiner simultanément à la substance (50) en différents sites dans la substance (50).

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