Detection method for influenza viruses

G - Physics – 01 – N

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G01N 33/569 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2652404

A method of rapid detection of influenza viruses and/or virus particles comprising a hemagglutinin and a neuraminidase component, said method comprising the steps of: a) binding the viruses and/or virus particles to a support containing at least one type of carbohydrate receptor selected from the group consisting of natural or synthetic oligosaccharide, which is conjugated to, or situated in composition with glycoproteins like glycophorin, al-acid glycoprotein, a2-macroglobulin, ovomucoid, and combinations thereof which carbohydrate receptor binds to the hemagglutinin component of the viruses and/or virus particles; b) reacting the neuraminidase component of the bound viruses and/or virus particles with its labelled enzyme substrate, causing the generation of a detectable signal; and c) detecting the signal generated in step b).

La présente invention concerne un procédé de détection rapide des virus de la grippe et/ou de particules de virus, comprenant un composant d'hémagglutinine et un autre de neuraminidase. Ledit procédé comprend les étapes suivantes: a) lier les virus et/ou les particules de virus à un support contenant au moins un type de récepteur de glucides sélectionné dans le groupe constitué par des oligosaccharides naturels ou synthétiques, qui est conjugué ou forme une composition avec des glycoprotéines du type glycophorine, al-glycoprotéine acide, a2-macroglobuline, ovomucoïde et leurs combinaisons, dans lesquelles le récepteur de glucides se lie au composant d'hémagglutinine des virus et/ou des particules de virus; b) faire réagir le composant de neuraminidase des virus et/ou des particules de virus liés avec son substrat enzymatique marqué, ce qui entraîne la génération d'un signal détectable; et c) détecter le signal généré à l'étape b).

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