Detection methods

Details Detection methods Detection methods

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2365565

The invention relates to a method for quantitatively or qualitatively detecting an analyte in a sample, with the sample being incubated, for the purpose of avoiding, diminishing and/or detecting the high-dose hook effect, with an analyte-specific binding partner R1, which is associated with a solid phase, an analyte--specific binding partner R2, which is associated with a label L1, and an analyte-specific binding partner R3, which is associated with a label L2, and the L1--dependent measurement signal being determined either at a different time from the L2-dependent or L1 plus L2--dependent measurement signal or using a different measurement method.

Méthode de détection quantitative ou qualitative d'un analyte dans un échantillon. Afin d'empêcher, de diminuer et/ou de détecter l'effet crochet, la méthode comprend l'incubation de l'échantillon avec un partenaire de liaison R1 spécifique à l'analyte, qui est associé à une phase solide, un partenaire de liaison R2 spécifique à l'analyte, qui est associé à un marqueur L1, et un partenaire de liaison R3 spécifique à l'analyte, qui est associé à un marqueur L2; le signal de mesure dépendant du marqueur L1 est déterminé soit à un moment différent du signal de mesure dépendant de L2 ou dépendant de L1 + L2, soit par une autre méthode de mesure.

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