Detection of agglutination of assays

G - Physics – 01 – N

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G01N 33/80 (2006.01) B01D 21/26 (2006.01) B01L 3/00 (2006.01) B01L 3/14 (2006.01) B04B 5/04 (2006.01) C12M 1/10 (2006.01) G01N 33/49 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2458827

An apparatus for conducting an assay having an agglutination or size separation step, includes: a first section disposed to receive a fluid to be assayed; and a second section disposed to receive the fluid from the first section upon application of a motive force, preferably centrifugal force, to the fluid, the second section comprising elements fixed to a substrate and adapted to mix the fluid and trap agglutinated particles. In a preferred embodiment, the elements are shaped as pillars. In another preferred embodiment, a third section is provided after the second section and the apparatus is in the form of a disk, preferably an optical disk, having a central axis, and wherein the first, second and third section are arranged in the disk as channels in a direction away from the central axis, respectively. A method for assaying a fluid that has particles to be separated or agglutinated includes: providing a fluid to be assayed into a first section of an apparatus as described above; applying a motive force to the fluid to move the fluid from the first section into a second section, wherein said second section comprises elements fixed to a substrate and adapted to mix the fluid and trap particles in the fluid; and measuring a property of the fluid. In a preferred embodiment, the fluid to be assayed is blood.

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