Detection of alzheimer's disease and other diseases using an...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/25 (2006.01) G01N 33/53 (2006.01) G01N 33/573 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2173993

An improved method for diagnosing a disease comprising detecting a disease-specific biochemical marker macromolecule within a sample of extracted cerebral spinal fluid or serum is disclosed. In particular, a radioactively labeled photoaffinity probe is used todiagnose a disease. For instance, Alzheimer's disease can be diagnosed by detecting a disease-specific protein having a molecular weight of about 42,000 daltons, i.e., glutamine synthetase. Also included is a method for detecting other disease states, such as cancer, by detecting the presence or absence of specific nucleotide binding proteins.

Procédé amélioré permettant de diagnostiquer une maladie, et consistant à détecter une macro-molécule marqueur biochimique spécifique d'une maladie dans un échantillon de sérum ou de liquide céphalo-rachidien extrait. En particulier, une sonde à photo-affinité à marquage radioactif est utilisée pour diagnostiquer une maladie. La maladie d'Alzheimer peut ainsi être diagnostiquée par la détection d'une protéine spécifique de cette maladie, et d'un poids moléculaire d'environ 42 000 daltons, en d'autres termes, la glutamine-synthétase. L'invention se rapporte également à un procédé de détection d'autres états pathologiques tels que le cancer, consistant à détecter la présence ou l'absence de protéines de liaison de nucléotides spécifiques.

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