Detection of amplified nucleic acid sequences using...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2226721

The invention describes an assay for detecting amplified target nucleic acid sequences with a visual signal. The sensitivity and specificity of the methodology are based on bifunctional target labeling during the amplification step or subsequent hybridization that generates a bifunctional label. The invention may be used, for example, in the screening of amplicon detection for the purpose of more efficiently screening libraries. The invention is also useful to detect nucleic acid sequences indicative of a genetic defect or contagious disease when used with the appropriate primers, as well as detect the existence of nucleic acid amplification.

La présente invention concerne une méthode destinée à la détection de séquences amplifiées d'acide nucléique cibles grâce à un signal visuel. La sensibilité et la spécificité de la méthode ont pour base le marquage bifonctionnel des cibles lors de l'opération d'amplification ou de l'hybridation subséquente, qui produit une marque bifonctionnelle. L'invention peut être utilisée, par exemple, dans le test de détection d'amplicon ayant pour but un tri plus efficace des bibliothèques. L'invention est également utile pour détecter les séquences d'acide nucléique indiquant un défaut génétique ou une maladie contagieuse, quand elle est utilisée avec les amorces voulues, ainsi que pour détecter l'existence de l'amplification de l'acide nucléique.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Detection of amplified nucleic acid sequences using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of amplified nucleic acid sequences using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of amplified nucleic acid sequences using... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1885469

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.