C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2075683
DETECTION OF AMPLIFIED NUCLEIC ACID USING SECONDARY CAPTURE OLIGONUCLEOTIDES AND TEST KIT Abstract of the Disclosure A target nucleic acid can be amplified and detected using a water-insoluble capture probe having a short oligonucleotide (15-20 nucleotides) covalently attached to a polymeric particle, and one or more secondary oligonucleotides. The capture probe and secondary oligonucleotides are complementary to the target nucleic acid, although the sequences of complementarity are different. Moreover, each of the secondary oligonucleotides has a length which is equal to or greater than the length of the capture probe oligonucleotide. The secondary oligonucleotides are hybridized to the target nucleic acid either before or during hybridization of the target to the capture probe. The result is improved hybridization efficiency of capture probe and target nucleic acid.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
Wu Annie Liu
LandOfFree
Detection of amplified nucleic acid using secondary capture... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of amplified nucleic acid using secondary capture..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of amplified nucleic acid using secondary capture... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2049532