G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/52 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2186743
The invention relates to an improved method of detecting analytes in a sample of body fluid. In this method, the sample is contacted with a specific binding agent capable of forming a specific binding complex with the analyte wherein the specific binding agent is immobilised on a porous solid substrate. The presence of specific binding complex is then detected. The method of the invention has the additional step of wiping the surface of the substrate before detection takes place in order to remove unadsorbed contaminants. The unadsorbed contaminants may be visualised on the surface of the substrate using a colouring agent so that it is easy to tell when the contaminants have been removed.
L'invention concerne un procédé amélioré de détection d'analyte dans un échantillon de liquide corporel. D'après ce procédé, on met l'échantillon en contact avec un agent de liaison spécifique pouvant constituer un complexe de liaison spécifique avec l'analyte, ledit agent étant immobilisé sur un substrat solide poreux. On détecte ensuite la présence du complexe de liaison spécifique. Dans une étape complémentaire, on essuie la surface du substrat préalablement à la détection, afin de supprimer les contaminants non adsorbés. On peut visualiser ces derniers sur la surface du substrat au moyen d'un agent colorant et il est, de ce fait, facile de déterminer le moment de la suppression desdits contaminants.
Cortecs Limited
Swabey Ogilvy Renault
LandOfFree
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