Detection of analytes by fluorescent lanthanide chelates

Details Detection of analytes by fluorescent lanthanide chelates Detection of analytes by fluorescent lanthanide chelates

G - Physics

01

N

G01N 33/58 (2006.01) G01N 33/52 (2006.01) G01N 33/66 (2006.01)

Patent

CA 2322858

Compositions and methods for determining the presence or concentration of an analyte in a sample by exposing the sample to an indicator molecule comprising a fluorescent lanthanide metal chelate complex. The presence or concentration of the analyte in the sample is determined by observing and/or measuring the change in intensity of fluorescence emitted by the lanthanide metal chelate complex upon binding of the analyte to one or more recognition elements in the complex. The fluorescent indicator molecules can be used in various types of fluorescent sensing devices and are useful in various fields, including energy, medicine and agriculture.

L'invention concerne des compositions et des méthodes permettant de détecter la présence ou la concentration d'un analyte dans un échantillon, les méthodes de cette invention consistant à exposer un échantillon à une molécule indicatrice renfermant un complexe chelaté de métal lanthanidique fluorescent. On peut détecter la présence ou la concentration de l'analyte dans ledit échantillon en observant et/ou en mesurant toute modification de l'intensité de la fluorescence émise par le complexe chelaté de métal lanthanidique fluorescent, une fois ledit analyte fixé à un ou plusieurs éléments de reconnaissance du complexe. Les molécules indicatrices fluorescentes peuvent être utilisées dans différents types de dispositifs de détection par fluorescence et dans de nombreux domaines, notamment l'énergie, la médecine, et l'agriculture.

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