Detection of anti-hepatitis b drug resistance

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01)

Patent

CA 2339422

The present invention relates to a method for the monitoring of anti-HBV drug resistance in a patient by genetic detection of at least one of the mutations L528M, M552V/I and/or V/L/M555I in the DNA polymerase of the HBV strains present in a biological sample of said patient. The present invention provides new HBV DNA polymerase sequences to be used for the design of new probes allowing a very specific and sensitive detection of anti-HBV drug resistance.

La présente invention concerne un procédé de surveillance de la résistance médicamenteuse anti-HBV chez un patient par détection génétique d'au moins une des mutations L528M, M552V/I et/ou V/L/M555I dans l'ADN-polymérase des souches HBV présentes dans un échantillon biologique dudit patient. L'invention concerne de nouvelles séquences d'ADN-polymérase HBV pouvant être utilisées lors de la conception de nouvelles sondes permettant une détection spécifique et efficace de la résistance médicamenteuse anti-HBV.

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