Detection of antibodies to gangliosides using solid-phase...

Details Detection of antibodies to gangliosides using solid-phase... Detection of antibodies to gangliosides using solid-phase...

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2378172

Method of detecting antibodies to one or more ganglioside(s) of interest in a sample are disclosed which comprise using a solid-phase reactant having carbonyl groups attached thereon, and the ganglioside(s) of interest linked to the solid-phase reactant by an amide bond between an amino group of ganglioside of interest and a carbonyl group attached to the solid-phase reactant. The methods of detecting antibodies to ganglioside(s) of interest can be used in methods of diagnosing neuropathies in an individual.

L'invention concerne des procédés permettant de détecter des anticorps dirigés contre un ou plusieurs ganglioside(s) donné(s) dans un échantillon. Ces procédés comprennent l'utilisation d'un réactif en phase solide sur lequel sont fixés des groupes carbonyle, les ganglioside(s) étant liés à ce réactif en phase solide par une liaison amide entre un groupe amino du ganglioside et un groupe carbonyle fixé sur le réactif en phase solide. Ces procédés de détection d'anticorps dirigés contre un/des ganglioside(s) donné(s) peuvent être utilisés dans des méthodes permettant le diagnostic de neuropathies chez un individu.

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